• Immunofluorescence analysis of paraffin-embedded mouse spinal cord using CNTF Rabbit polyclonal antibody (STJ23186) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using CNTF Rabbit polyclonal antibody (STJ23186) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from mouse muscle, using CNTF Rabbit polyclonal antibody (STJ23186) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CNTF antibody (24-74) (STJ23186)

SKU:
STJ23186

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CNTF (24-74) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CNTF
Gene ID: 1270
Uniprot ID: CNTF_HUMAN
Immunogen Region: 24-74
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 24-74 of human CNTF (NP_000605.1).
Immunogen Sequence: ARKIRSDLTALTESYVKHQG LNKNINLDSADGMPVASTDQ WSELTEAERLQ
Tissue Specificity Nervous system.
Function CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Protein Name Ciliary Neurotrophic Factor
Cntf
Database Links Reactome: R-HSA-6788467
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ciliary Neurotrophic Factor antibody
Anti-Cntf antibody
Anti-CNTF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance