• Western blot analysis of extracts of various cell lines, using CMPK1 antibody (STJ28644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of MCF7 cells using CMPK1 antibody (STJ28644). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of HT-29 cells using 3 Mu g CMPK1 antibody (STJ28644). Western blot was performed from the immunoprecipitate using CMPK1 antibody (STJ28644) at a dilution of 1:1000.

Anti-CMPK1 antibody (69-228) (STJ28644)

SKU:
STJ28644

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/IP/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CMPK1
Gene ID: 51727
Uniprot ID: KCY_HUMAN
Immunogen Region: 69-228
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 69-228 of human CMPK1 (NP_057392.1).
Immunogen Sequence: LLRDERKNPDSQYGELIEKY IKEGKIVPVEITISLLKREM DQTMAANAQKNKFLIDGFPR NQDNLQGWNKTMDGKADVSF VLFFDCNNEICIERCLERGK SSGRSDDNRESLEKRIQTYL QSTKPIIDLYEEMGKVKKID ASKSVDEVFDEVVQIFDKEG
Tissue Specificity Ubiquitously expressed.
Function Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity.
Protein Name Ump-Cmp Kinase
Deoxycytidylate Kinase
Ck
Dcmp Kinase
Nucleoside-Diphosphate Kinase
Uridine Monophosphate/Cytidine Monophosphate Kinase
Ump/Cmp Kinase
Ump/Cmpk
Database Links Reactome: R-HSA-499943
Cellular Localisation Nucleus
Cytoplasm
Predominantly Cytoplasmic
Less Than 15% Nuclear
Alternative Antibody Names Anti-Ump-Cmp Kinase antibody
Anti-Deoxycytidylate Kinase antibody
Anti-Ck antibody
Anti-Dcmp Kinase antibody
Anti-Nucleoside-Diphosphate Kinase antibody
Anti-Uridine Monophosphate/Cytidine Monophosphate Kinase antibody
Anti-Ump/Cmp Kinase antibody
Anti-Ump/Cmpk antibody
Anti-CMPK1 antibody
Anti-CMK antibody
Anti-CMPK antibody
Anti-UCK antibody
Anti-UMK antibody
Anti-UMPK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance