• Immunohistochemistry analysis of paraffin-embedded mouse fetal liver using CMIP Rabbit polyclonal antibody (STJ11103339) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of K-562 cells, using CMIP antibody (STJ11103339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using CMIP antibody (STJ11103339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CMIP antibody (640-773) (STJ11103339)

SKU:
STJ11103339

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CMIP (640-773) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CMIP
Gene ID: 80790
Uniprot ID: CMIP_HUMAN
Immunogen Region: 640-773
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 640-773 of human CMIP (NP_938204.2).
Immunogen Sequence: RLTLPSKSTDADLARLLSSG SFGNLENLSLAFTNVTSACA EHLIKLPSLKQLNLWSTQFG DAGLRLLSEHLTMLQVLNLC ETPVTDAGLLALSSMKSLCS LNMNSTKLSADTYEDLKAKL PNLKEVDVRYTEAW
Tissue Specificity Isoform 1 is expressed in peripheral blood mononuclear cells and kidney. Lower expression in brain and liver. Expression is down-regulated in activated cells. Isoform 2 is expressed in lymphocyte precursors, however, expression shuts down during maturation and differentiation in thymus and fetal liver.
Function Plays a role in T-cell signaling pathway. Isoform 2 may play a role in T-helper 2 (Th2) signaling pathway and seems to represent the first proximal signaling protein that links T-cell receptor-mediated signal to the activation of c-Maf Th2 specific factor.
Protein Name C-Maf-Inducing Protein
C-Mip
Truncated C-Maf-Inducing Protein
Tc-Mip
Cellular Localisation Nucleus
Cytoplasm
Isoform 2 Is Translocated To The Nucleus And Is Specifically Recruited During Minimal Change Nephrotic Syndrome (Mcns)
Detected In Nuclear And Cytoplasmic Compartments During Mcns Relapse
Expressed In Cytoplasm Only During Mcns Remission And Absent In Normal Patients
Alternative Antibody Names Anti-C-Maf-Inducing Protein antibody
Anti-C-Mip antibody
Anti-Truncated C-Maf-Inducing Protein antibody
Anti-Tc-Mip antibody
Anti-CMIP antibody
Anti-KIAA1694 antibody
Anti-TCMIP antibody

Information sourced from Uniprot.org

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