• STJ73485 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ73485 Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0. 4ug/ml) showing weak staining. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-CMG1/CCDC2/IFT74 antibody (STJ73485)

SKU:
STJ73485-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CMG1/CCDC2/IFT74 is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm and Golgi apparatus of U251 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: IFT74
Gene ID: 80173
Uniprot ID: IFT74_HUMAN
Accession Number: NP_001092692.1; NP_001092693.1; NP_079379.2; AAK77221.1
Immunogen Sequence: CKTIVDALHSTSGN
Function Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis. Essential for flagellogenesis during spermatogenesis.
Protein Name Intraflagellar Transport Protein 74 Homolog
Capillary Morphogenesis Gene 1 Protein
Cmg-1
Coiled-Coil Domain-Containing Protein 2
Database Links Reactome: R-HSA-5620924
Cellular Localisation Cell Projection
Cilium
Cytoplasmic Vesicle
Flagellum
Secretory Vesicle
Acrosome
Localizes Along Primary Cilia At Interphase And Around The Basal Body/Centriole At Interphase And Mitosis
In Male Germ Cells
Strongly Expressed In The Vesicles Of Spermatocytes And Round Spermatids And Also In The Acrosome And Centrosome Regions Of Elongating Spermatids And In Developing Sperm Tails
Alternative Antibody Names Anti-Intraflagellar Transport Protein 74 Homolog antibody
Anti-Capillary Morphogenesis Gene 1 Protein antibody
Anti-Cmg-1 antibody
Anti-Coiled-Coil Domain-Containing Protein 2 antibody
Anti-IFT74 antibody
Anti-CCDC2 antibody
Anti-CMG1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance