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Anti-CLTC antibody (200-300) [S3MR] (STJ11102203)

Immunoprecipitation analysis of 300 Mu g extracts of NIH/3T3 cells using 3 Mu g Clathrin heavy chain antibody (STJ11102203). Western blot was performed from the immunoprecipitate using Clathrin heavy chain antibody (STJ11102203) at a dilution of 1:1000.
Confocal imaging of HeLa cells using Clathrin heavy chain Rabbit monoclonal antibody (STJ11102203, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Rabbit monoclonal antibody ( dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
Immunohistochemistry analysis of Clathrin heavy chain in paraffin-embedded human thyroid cancer tissue using Clathrin heavy chain Rabbit monoclonal antibody (STJ11102203) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of Clathrin heavy chain in paraffin-embedded human colon tissue using Clathrin heavy chain Rabbit monoclonal antibody (STJ11102203) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of Clathrin heavy chain in paraffin-embedded human colon carcinoma tissue using Clathrin heavy chain Rabbit monoclonal antibody (STJ11102203) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
Western blot analysis of various lysates using Clathrin heavy chain Rabbit monoclonal antibody (STJ11102203) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CLTC antibody (200-300) [S3MR] (STJ11102203)

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STJ11102203

£146.50 - £639.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/IP
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-Clathrin heavy chain (200-300) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.

Clonality:	Monoclonal
Clone ID:	S3MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200 IP 1:500-1:1000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	CLTC
Gene ID:	1213
Uniprot ID:	CLH1_HUMAN
Immunogen Region:	200-300
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Clathrin heavy chain (Q00610).
Immunogen Sequence:	SFAQFKMEGNAEESTLFCFA VRGQAGGKLHIIEVGTPPTG NQPFPKKAVDVFFPPEAQND FPVAMQISEKHDVVFLITKY GYIHLYDLETGTCIYMNRIS G

Function	Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation.
Protein Name	Clathrin Heavy Chain 1 Clathrin Heavy Chain On Chromosome 17 Clh-17
Database Links	Reactome: R-HSA-168275 Reactome: R-HSA-177504 Reactome: R-HSA-190873 Reactome: R-HSA-196025 Reactome: R-HSA-2132295 Q00610-1 Reactome: R-HSA-3928665 Reactome: R-HSA-432720 Q00610-1 Reactome: R-HSA-432722 Q00610-1 Reactome: R-HSA-437239 Reactome: R-HSA-5099900 Reactome: R-HSA-5140745 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 Reactome: R-HSA-8866427 Reactome: R-HSA-8964038 Reactome: R-HSA-9013420 Reactome: R-HSA-9013424 Reactome: R-HSA-9700645 Reactome: R-HSA-9725370 Reactome: R-HSA-9725371
Cellular Localisation	Cytoplasmic Vesicle Membrane Peripheral Membrane Protein Cytoplasmic Side Membrane Coated Pit Melanosome Cytoplasm Cytoskeleton Spindle Cytoplasmic Face Of Coated Pits And Vesicles Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv In Complex With Tacc3 And Ckap5 (Forming The Tacc3/Ch-Tog/Clathrin Complex) Localized To Inter-Microtubule Bridges In Mitotic Spindles
Alternative Antibody Names	Anti-Clathrin Heavy Chain 1 antibody Anti-Clathrin Heavy Chain On Chromosome 17 antibody Anti-Clh-17 antibody Anti-CLTC antibody Anti-CLH17 antibody Anti-CLTCL2 antibody Anti-KIAA0034 antibody

Information sourced from Uniprot.org

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