• Immunoprecipitation analysis of 300 Mu g extracts of NIH/3T3 cells using 3 Mu g Clathrin heavy chain antibody (STJ11102203). Western blot was performed from the immunoprecipitate using Clathrin heavy chain antibody (STJ11102203) at a dilution of 1:1000.
  • Confocal imaging of HeLa cells using Clathrin heavy chain Rabbit monoclonal antibody (STJ11102203, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Rabbit monoclonal antibody ( dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Immunohistochemistry analysis of Clathrin heavy chain in paraffin-embedded human thyroid cancer tissue using Clathrin heavy chain Rabbit monoclonal antibody (STJ11102203) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Clathrin heavy chain in paraffin-embedded human colon tissue using Clathrin heavy chain Rabbit monoclonal antibody (STJ11102203) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Clathrin heavy chain in paraffin-embedded human colon carcinoma tissue using Clathrin heavy chain Rabbit monoclonal antibody (STJ11102203) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Clathrin heavy chain Rabbit monoclonal antibody (STJ11102203) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CLTC antibody (200-300) [S3MR] (STJ11102203)

SKU:
STJ11102203

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:200-1:2000
IF/ICC:1:100-1:400
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your sp
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLTCL1
CLTC
Gene ID: 8218
1213
Uniprot ID: CLH2_HUMAN
CLH1_HUMAN
Immunogen Region: 200-300
Specificity: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Clathrin heavy chain (Q00610).
Immunogen Sequence: SFAQFKMEGNAEESTLFCFA VRGQAGGKLHIIEVGTPPTG NQPFPKKAVDVFFPPEAQND FPVAMQISEKHDVVFLITKY GYIHLYDLETGTCIYMNRIS G
Tissue Specificity
Alternative Antibody Names Anti-Clathrin Heavy Chain 2 antibody
Anti-Clathrin Heavy Chain On Chromosome 22 antibody
Anti-Clh-22 antibody
Anti-CLTCL1 antibody
Anti-CLH22 antibody
Anti-CLTCL antibody
Anti-CLTD antibody
Anti-Clathrin Heavy Chain 1 antibody
Anti-Clathrin Heavy Chain On Chromosome 17 antibody
Anti-Clh-17 antibody
Anti-CLTC antibody
Anti-CLH17 antibody
Anti-CLTCL2 antibody
Anti-KIAA0034 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance