Anti-CLK2 antibody (239-498) (STJ110195)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CLK2 (239-498) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CLK2 |
| Gene ID | 1196 |
| Uniprot ID | CLK2_HUMAN |
| Immunogen Region | 239-498 |
| Immunogen Sequence | CISFELLGLSTFDFLKDNNY LPYPIHQVRHMAFQLCQAVK FLHDNKLTHTDLKPENILFV NSDYELTYNLEKKRDERSVK STAVRVVDFGSATFDHEHHS TIVSTRHYRAPEVILELGWS QPCDVWSIGCIIFEYYVGFT LFQTHDNREHLAMMERILGP IPSRMIRKTRKQKYFYRGRL DWDENTSAGRYVRENCKPLR RYLTSEAEEHHQLFDLIESM LEYEPAKRLTLGEALQHPF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 239-498 of human CLK2 (NP_003984.2). |
Additional Info
| Tissue Specificity | Endothelial cells. Expressed in androgen-dependent prostate cancer cells. |
| Post Translational Modifications | Autophosphorylates on all three types of residues. Phosphorylation on Ser-34 and Thr-127 by AKT1 is induced by ionizing radiation or insulin. Phosphorylation plays a critical role in cell proliferation following low dose radiation and prevents cell death following high dose radiation. Phosphorylation at Thr-344 by PKB/AKT2 induces its kinase activity which is required for its stability. The phosphorylation status at Ser-142 influences its subnuclear localization.inhibition of phosphorylation at Ser-142 results in accumulation in the nuclear speckle. |
| Function | Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Phosphorylates PAGE4 at several serine and threonine residues and this phosphorylation attenuates the ability of PAGE4 to potentiate the transcriptional activator activity of JUN. |
| Protein Name | Dual Specificity Protein Kinase Clk2Cdc-Like Kinase 2 |
| Database Links | |
| Cellular Localisation | NucleusIsoform 1: NucleusNucleus SpeckleInhibition Of Phosphorylation At Ser-142 Results In Accumulation In The Nuclear SpeckleIsoform 2: Nucleus SpeckleCo-Localizes With Serine- And Arginine-Rich (Sr) Proteins In The Nuclear Speckles |
| Alternative Antibody Names | Anti-Dual Specificity Protein Kinase Clk2 antibodyAnti-Cdc-Like Kinase 2 antibodyAnti-CLK2 antibody |
Information sourced from Uniprot.org