| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CLK2 (239-498) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CLK2 |
| Gene ID: |
1196 |
| Uniprot ID: |
CLK2_HUMAN |
| Immunogen Region: |
239-498 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 239-498 of human CLK2 (NP_003984.2). |
| Immunogen Sequence: |
CISFELLGLSTFDFLKDNNY LPYPIHQVRHMAFQLCQAVK FLHDNKLTHTDLKPENILFV NSDYELTYNLEKKRDERSVK STAVRVVDFGSATFDHEHHS TIVSTRHYRAPEVILELGWS QPCDVWSIGCIIFEYYVGFT LFQTHDNREHLAMMERILGP IPSRMIRKTRKQKYFYRGRL DWDENTSAGRYVRENCKPLR RYLTSEAEEHHQLFDLIESM LEYEPAKRLTLGEALQHPF |
| Tissue Specificity | Endothelial cells. Expressed in androgen-dependent prostate cancer cells. |
| Post Translational Modifications | Autophosphorylates on all three types of residues. Phosphorylation on Ser-34 and Thr-127 by AKT1 is induced by ionizing radiation or insulin. Phosphorylation plays a critical role in cell proliferation following low dose radiation and prevents cell death following high dose radiation. Phosphorylation at Thr-344 by PKB/AKT2 induces its kinase activity which is required for its stability. The phosphorylation status at Ser-142 influences its subnuclear localization.inhibition of phosphorylation at Ser-142 results in accumulation in the nuclear speckle. |
| Function | Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Phosphorylates PAGE4 at several serine and threonine residues and this phosphorylation attenuates the ability of PAGE4 to potentiate the transcriptional activator activity of JUN. |
| Protein Name | Dual Specificity Protein Kinase Clk2Cdc-Like Kinase 2 |
| Cellular Localisation | NucleusIsoform 1: NucleusNucleus SpeckleInhibition Of Phosphorylation At Ser-142 Results In Accumulation In The Nuclear SpeckleIsoform 2: Nucleus SpeckleCo-Localizes With Serine- And Arginine-Rich (Sr) Proteins In The Nuclear Speckles |
| Alternative Antibody Names | Anti-Dual Specificity Protein Kinase Clk2 antibodyAnti-Cdc-Like Kinase 2 antibodyAnti-CLK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
