• STJ70503 (2µg/ml) staining of nuclear HEK293 (A) and (1ug/ml) Human Breast cancer (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70503 (1µg/ml) staining of Mouse (A) and (2ug/ml) Pig (B) Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70503 (10µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
  • STJ70503 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70503 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Clik1/STK35 antibody (C-Term) (STJ70503)

SKU:
STJ70503-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Clik1/STK35 (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-10µg/ml
IF-Strong expression of the protein seen in the nuclei of U2OS cells. 10µg/ml
FC-Flow cytometric analysis of HEK293 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: STK35
Gene ID: 140901
Uniprot ID: STK35_HUMAN
Immunogen Region: C-Term
Accession Number: NP_543026.2
Immunogen Sequence: ELETRMDQVTCAA
Post Translational Modifications Autophosphorylated.
Protein Name Serine/Threonine-Protein Kinase 35
Clp-36-Interacting Kinase 1
Clik-1
Pdlim1-Interacting Kinase 1
Serine/Threonine-Protein Kinase 35 L1
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
When Associated With Pdlim1
It Is Mostly Found In Cytoplasm
Localized To Actin Stress Fibers
However
Pubmed:19756140 Detected Stk35 Only In The Nucleus
And The Presence Of Pdlim1 Had No Influence On Its Location
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase 35 antibody
Anti-Clp-36-Interacting Kinase 1 antibody
Anti-Clik-1 antibody
Anti-Pdlim1-Interacting Kinase 1 antibody
Anti-Serine/Threonine-Protein Kinase 35 L1 antibody
Anti-STK35 antibody
Anti-CLIK1 antibody
Anti-PDIK1 antibody
Anti-STK35L1 antibody

Information sourced from Uniprot.org

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