Anti-Cleaved-PLG-V98 antibody (79-128 aa) (STJ90102)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human PLMN at the amino acid range 79-128
General Information
| Short Description | Rabbit polyclonal anti-Cleaved-Plasminogen-V98 (79-128 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PLG |
| Gene ID | 5340 |
| Uniprot ID | PLMN_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human PLMN at the amino acid range 79-128 |
| Immunogen Region | 79-128 aa |
| Specificity | Cleaved-Plasminogen HC A short form (V98) Polyclonal Antibody detects endogenous levels of fragment of activated Plasminogen HC A short form protein resulting from cleavage adjacent to V98. |
Additional Info
| Post Translational Modifications | N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity). In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide. (Microbial infection) The Y.pestis Pla protein cleaves between Arg-580 and Val-581, generating plasmin which facilitates bacterial migration and infection. |
| Function | Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1, C4 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. (Microbial infection) ENO/enoloase from parasite P.falciparum (strain NF54) interacts with PLG present in the mosquito blood meal to promote the invasion of the mosquito midgut by the parasite ookinete. The catalytic active form, plasmin, is essential for the invasion of the mosquito midgut. (Microbial infection) Binds to OspC on the surface of B.burgdorferi cells, possibly conferring an extracellular protease activity on the bacteria that allows it to traverse host tissue. (Microbial infection) Interacts with dengue virus type 2 particles. Enhances dengue virus type 2 infection in Aedes aegypti mosquito midgut by increasing midgut internalization, resulting in higher infection rates and viral dissemination in mosquitoes. |
| Protein Name | Plasminogen Cleaved Into - Plasmin Heavy Chain A - Activation Peptide - Angiostatin - Plasmin Heavy Chain A - Short Form - Plasmin Light Chain B |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-186797Reactome: R-HSA-381426Reactome: R-HSA-75205 |
| Cellular Localisation | SecretedLocates To The Cell Surface Where It Is Proteolytically Cleaved To Produce The Active PlasminInteraction With Hrg Tethers It To The Cell Surface |
| Alternative Antibody Names | Anti-Plasminogen Cleaved Into - Plasmin Heavy Chain A - Activation Peptide - Angiostatin - Plasmin Heavy Chain A - Short Form - Plasmin Light Chain B antibodyAnti-PLG antibody |
Information sourced from Uniprot.org