| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Cleaved-Neurogenic locus notch homolog protein 2-A1734 (1715-1764 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:10000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NOTCH2 |
| Gene ID: |
4853 |
| Uniprot ID: |
NOTC2_HUMAN |
| Immunogen Region: |
1715-1764 aa |
| Specificity: |
Cleaved-Notch 2 (A1734) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 2 protein resulting from cleavage adjacent to A1734. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human NOTCH2 at the amino acid range 1715-1764 |
| Post Translational Modifications | Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Hydroxylated by HIF1AN. Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1. Phosphorylated by GSK3. GSK3-mediated phosphorylation is necessary for NOTCH2 recognition by FBXW7, ubiquitination and degradation via the ubiquitin proteasome pathway. |
| Function | Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells. |
| Protein Name | Neurogenic Locus Notch Homolog Protein 2Notch 2Hn2 Cleaved Into - Notch 2 Extracellular TruncationN2ecd - Notch 2 Intracellular DomainN2icd |
| Database Links | Reactome: R-HSA-1912399Reactome: R-HSA-1912408Reactome: R-HSA-1912420Reactome: R-HSA-2197563Reactome: R-HSA-2979096Reactome: R-HSA-350054Reactome: R-HSA-5083630Reactome: R-HSA-9013695 |
| Cellular Localisation | Notch 2 Extracellular Truncation: Cell MembraneSingle-Pass Type I Membrane ProteinNotch 2 Intracellular Domain: NucleusCytoplasmFollowing Proteolytical Processing Nicd Is Translocated To The NucleusRetained At The Cytoplasm By Tcim |
| Alternative Antibody Names | Anti-Neurogenic Locus Notch Homolog Protein 2 antibodyAnti-Notch 2 antibodyAnti-Hn2 Cleaved Into - Notch 2 Extracellular Truncation antibodyAnti-N2ecd - Notch 2 Intracellular Domain antibodyAnti-N2icd antibodyAnti-NOTCH2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
