• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Cleaved-MMP1-Ile271 antibody (270-271) (STJA0005888)

SKU:
STJA0005888

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cleaved-Interstitial collagenase-Ile271 (270-271) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-p 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP1
Gene ID: 4312
Uniprot ID: MMP1_HUMAN
Immunogen Region: 270-271
Specificity: This antibody detects endogenous levels of Human MMP1 (Cleaved-Ile271, protein was cleaved amino acid sequence between 270-271 )
Immunogen: Synthesized peptide derived from human MMP1 (Cleaved-Ile271)
Post Translational Modifications Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Tyrosine phosphorylated in platelets by PKDCC/VLK.
Function Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
Protein Name Interstitial Collagenase
Fibroblast Collagenase
Matrix Metalloproteinase-1
Mmp-1 Cleaved Into - 22 Kda Interstitial Collagenase - 27 Kda Interstitial Collagenase
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-210991
Reactome: R-HSA-381426
Reactome: R-HSA-6785807
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Interstitial Collagenase antibody
Anti-Fibroblast Collagenase antibody
Anti-Matrix Metalloproteinase-1 antibody
Anti-Mmp-1 Cleaved Into - 22 Kda Interstitial Collagenase - 27 Kda Interstitial Collagenase antibody
Anti-MMP1 antibody
Anti-CLG antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance