| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Cleaved-Caspase-4 and Caspase-5-D270/D311 (221-270 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:40000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CASP5CASP4 |
| Gene ID: |
838837 |
| Uniprot ID: |
CASP5_HUMANCASP4_HUMAN |
| Immunogen Region: |
221-270 aa |
| Specificity: |
Cleaved-Caspase-4/5 p20 (D270/D311) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-4/5 p20 protein resulting from cleavage adjacent to D270/D311. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human Caspase 4/5 at the amino acid range 221-270 |
| Post Translational Modifications | In response to activation signals, including endoplasmic reticulum stress or treatment with amyloid-beta A4 protein fragments (such as amyloid-beta protein 40), undergoes autoproteolytic cleavage. |
| Function | Inflammatory caspase that acts as an essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS. Thiol protease that cleaves a tetrapeptide after an Asp residue at position P1. Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, followed by IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators. Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection: in later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which catalyzes cleavage of GSDMD, resulting in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion.this activity may not require caspase enzymatic activity. Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains. Activated by direct binding to LPS without the need of an upstream sensor. Does not directly process IL1B. During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation. |
| Protein Name | Caspase-4Casp-4Ice And Ced-3 Homolog 2Ich-2Ice(Rel-IiMih1Protease Tx Cleaved Into - Caspase-4 Subunit P10 - Caspase-4 Subunit P20 |
| Database Links | Reactome: R-HSA-168638 |
| Cellular Localisation | CytoplasmCytosolEndoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic SideMitochondrionInflammasomeSecretedPredominantly Localizes To The Endoplasmic Reticulum (Er)Association With The Er Membrane Requires Tmem214Released In The Extracellular Milieu By Keratinocytes Following Uvb Irradiation |
| Alternative Antibody Names | Anti-Caspase-4 antibodyAnti-Casp-4 antibodyAnti-Ice And Ced-3 Homolog 2 antibodyAnti-Ich-2 antibodyAnti-Ice(Rel-Ii antibodyAnti-Mih1 antibodyAnti-Protease Tx Cleaved Into - Caspase-4 Subunit P10 - Caspase-4 Subunit P20 antibodyAnti-CASP4 antibodyAnti-ICH2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
