Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Claudin-5 Polyclonal Antibody was diluted at 1:200 (4ยฐC, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98ยฐC, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-CLDN5 antibody (169-218 aa) (STJ92318)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Claudin 5 at the amino acid range 169-218
STJ92318
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General Information

Short DescriptionRabbit polyclonal anti-Claudin-5 (169-218 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
IF 1:100-300
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCLDN5
Gene ID7122
Uniprot IDCLD5_HUMAN
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Claudin 5 at the amino acid range 169-218
Immunogen Region169-218 aa
SpecificityClaudin-5 Polyclonal Antibody detects endogenous levels of Claudin-5 protein.

Additional Info

Protein Name Claudin-5
Transmembrane Protein Deleted In Vcfs
Tmdvcf
Database Links Reactome: R-HSA-420029
Reactome: R-HSA-8935964
Cellular Localisation Cell Junction
Tight Junction
Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Claudin-5 antibody
Anti-Transmembrane Protein Deleted In Vcfs antibody
Anti-Tmdvcf antibody
Anti-CLDN5 antibody
Anti-AWAL antibody
Anti-TMVCF antibody

Information sourced from Uniprot.org

Citations

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