• Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Claudin 3 (ABT-CLD3) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MCF7Predicted band size: 23kDaObserved band size: 18kDa
  • Human colon carcinoma tissue was stained with Anti-Claudin 3 (ABT-CLD3) Antibody
  • Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Claudin 3 (ABT-CLD3) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: SW480 Lane 2: MCF7Predicted band size: 23kDaObserved band size: 20kDa
  • Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Intestine. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Intestine. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Rectum. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Rectum. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of Claudin 3Antibody at 1:1000 dilution.

Anti-CLDN3 antibody (150-220 aa) [ABT-CLD3] (STJ196828)

SKU:
STJ196828

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/IF/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Claudin-3 (150-220 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot research applications.
Clonality: Monoclonal
Clone ID: ABT-CLD3
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:500-2000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLDN3
Gene ID: 1365
Uniprot ID: CLD3_HUMAN
Immunogen Region: 150-220 aa
Specificity: This antibody detects endogenous levels of human Claudin 3. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section. The antib
Immunogen: Synthesized peptide derived from the human Claudin 3 at the amino acid range 150-220
Protein Name Claudin-3
Clostridium Perfringens Enterotoxin Receptor 2
Cpe-R 2
Cpe-Receptor 2
Rat Ventral Prostate.1 Protein Homolog
Hrvp1
Database Links Reactome: R-HSA-420029
Cellular Localisation Cell Junction
Tight Junction
Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Claudin-3 antibody
Anti-Clostridium Perfringens Enterotoxin Receptor 2 antibody
Anti-Cpe-R 2 antibody
Anti-Cpe-Receptor 2 antibody
Anti-Rat Ventral Prostate.1 Protein Homolog antibody
Anti-Hrvp1 antibody
Anti-CLDN3 antibody
Anti-C7orf1 antibody
Anti-CPETR2 antibody

Information sourced from Uniprot.org

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