| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CLCN2 (400-450 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Clcn2 |
| Gene ID: |
12724 |
| Uniprot ID: |
CLCN2_MOUSE |
| Immunogen Region: |
400-450 aa |
| Specificity: |
Specific for CLCN2. |
| Immunogen: |
A synthetic peptide from aa region 400-450 of mouse CLCN2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human. |
| Post Translational Modifications | Phosphorylated. Activated by dephosphorylation. |
| Function | Voltage-gated and osmosensitive chloride channel. Forms a homodimeric channel where each subunit has its own ion conduction pathway. Conducts double-barreled currents controlled by two types of gates, two fast glutamate gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Displays inward rectification currents activated upon membrane hyperpolarization and extracellular hypotonicity. Contributes to chloride conductance involved in neuron excitability. In hippocampal neurons, generates a significant part of resting membrane conductance and provides an additional chloride efflux pathway to prevent chloride accumulation in dendrites upon GABA receptor activation. In glia, associates with the auxiliary subunit HEPACAM/GlialCAM at astrocytic processes and myelinated fiber tracts where it may regulate transcellular chloride flux buffering extracellular chloride and potassium concentrations. Regulates aldosterone production in adrenal glands. The opening of CLCN2 channels at hyperpolarized membrane potentials in the glomerulosa causes cell membrane depolarization, activation of voltage-gated calcium channels and increased expression of aldosterone synthase, the rate-limiting enzyme for aldosterone biosynthesis. Contributes to chloride conductance in retinal pigment epithelium involved in phagocytosis of shed photoreceptor outer segments and photoreceptor renewal. Conducts chloride currents at the basolateral membrane of epithelial cells with a role in chloride reabsorption rather than secretion. Permeable to small monovalent anions with chloride > thiocyanate > bromide > nitrate > iodide ion selectivity. |
| Protein Name | Chloride Channel Protein 2Clc-2 |
| Database Links | Reactome: R-MMU-2672351 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinMyelin MembraneBasolateral Cell MembraneCell ProjectionDendritic Spine MembraneAxonSorting To The Basolateral Membrane Is Mediated By Ap-1 Clathrin AdapterLocalizes At Axon Initial Segments And Dendritic Shaft And SpikesColocalizes With Hepacam And Gfap At Astrocyte End-Foot In Contact With Brain Capillaries And Other Glial Cells |
| Alternative Antibody Names | Anti-Chloride Channel Protein 2 antibodyAnti-Clc-2 antibodyAnti-Clcn2 antibodyAnti-Clc2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
