• Immunohistochemistry analysis of CLCC1 in paraffin-embedded Human colon carcinoma using CLCC1 Rabbit polyclonal antibody (STJ119709) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CLCC1 Rabbit polyclonal antibody (STJ119709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CLCC1 antibody (302-501) (STJ119709)

SKU:
STJ119709

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLCC1 (302-501) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLCC1
Gene ID: 23155
Uniprot ID: CLCC1_HUMAN
Immunogen Region: 302-501
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 302-501 of human CLCC1 (NP_055942.1).
Immunogen Sequence: GAGKSVHVLRHIGGPESEPP QALRPRDRRRQEEIDYRPDG GAGDADFHYRGQMGPTEQGP YAKTYEGRREILRERDVDLR FQTGNKSPEVLRAFDVPDAE AREHPTVVPSHKSPVLDTKP KETGGILGEGTPKESSTESS QSAKPVSGQDTSGNTEGSPA AEKAQLKSEAAGSPDQGSTY SPARGVAGPRGQDPVSSPCG
Tissue Specificity Expressed in the retina of the eye, with extensive expression in the lamina cribrosa, optic nerve, ganglion cell layer, inner nuclear layer, outer nuclear layer and retinal pigment epithelium.
Function Seems to act as a chloride ion channel. Plays a role in retina development.
Protein Name Chloride Channel Clic-Like Protein 1
Mid-1-Related Chloride Channel Protein 1
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Golgi Apparatus Membrane
Nucleus Membrane
Within The Endoplasmic Reticulum (Er)
Localizes To The Mitochondria-Associated Er Membrane
A Zone Of Contact Between The Er And Mitochondrial Membranes
Alternative Antibody Names Anti-Chloride Channel Clic-Like Protein 1 antibody
Anti-Mid-1-Related Chloride Channel Protein 1 antibody
Anti-CLCC1 antibody
Anti-KIAA0761 antibody
Anti-MCLC antibody

Information sourced from Uniprot.org

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