| Host: | Rabbit |
| Applications: | WB/IHC-P |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal anti-Clathrin Heavy Chain for use in WB and IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | R02-6C4 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: | Affinity Purified |
| Concentration: | 0.3 mg/mL |
| Dilution Range: | WB 1:500-1:1000IHC 1:50-1:100 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | CLTC |
| Gene ID: | 1213 |
| Uniprot ID: | CLH1_HUMAN |
| Immunogen: | A synthetic peptide of human Clathrin heavy chain |
| Function | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation. Interaction with DNAJC6 mediates the recruitment of HSPA8 to the clathrin lattice and creates local destabilization of the lattice promoting uncoating. |
| Protein Name | Clathrin Heavy Chain 1Clathrin Heavy Chain On Chromosome 17Clh-17 |
| Database Links | Reactome: R-HSA-168275Reactome: R-HSA-177504Reactome: R-HSA-190873Reactome: R-HSA-196025Reactome: R-HSA-2132295 Q00610-1Reactome: R-HSA-3928665Reactome: R-HSA-432720 Q00610-1Reactome: R-HSA-432722 Q00610-1Reactome: R-HSA-437239Reactome: R-HSA-5099900Reactome: R-HSA-5140745Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8866427Reactome: R-HSA-8964038Reactome: R-HSA-9013420Reactome: R-HSA-9013424Reactome: R-HSA-9700645Reactome: R-HSA-9725370 |
| Cellular Localisation | Cytoplasmic Vesicle MembranePeripheral Membrane ProteinCytoplasmic SideMembraneCoated PitMelanosomeCytoplasmCytoskeletonSpindleCytoplasmic Face Of Coated Pits And VesiclesIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvIn Complex With Tacc3 And Ckap5 (Forming The Tacc3/Ch-Tog/Clathrin Complex) Localized To Inter-Microtubule Bridges In Mitotic Spindles |
| Alternative Antibody Names | Anti-Clathrin Heavy Chain 1 antibodyAnti-Clathrin Heavy Chain On Chromosome 17 antibodyAnti-Clh-17 antibodyAnti-CLTC antibodyAnti-CLH17 antibodyAnti-CLTCL2 antibodyAnti-KIAA0034 antibody |
Information sourced from Uniprot.org