| Host: |
Rat |
| Applications: |
WB/ICC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rat monoclonal antibody anti-CLASP2 (N-Term) is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
KT68 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2b |
| Formulation: |
PBS + 0.05% NaN3 |
| Purification: |
Protein G Purified |
| Dilution Range: |
WB 1:1000IHCICC 1:200IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Clasp2 |
| Gene ID: |
76499 |
| Uniprot ID: |
CLAP2_MOUSE |
| Immunogen Region: |
N-Term |
| Specificity: |
The antibody detects a 160 kDa* doublet corresponding to the molecular mass of CLASP2 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects CLASP2 by immunocytochemistry in HeLa and A431 cells. |
| Immunogen: |
Clone KT68 was generated from a GST fusion protein containing the N-terminus of mouse CLASP2. This sequence is highly conserved in human and rat CLASP2, and has low homology to CLASP1. |
| Tissue Specificity | Highly expressed in brain and at low levels in heart, kidney and lung. |
| Post Translational Modifications | Phosphorylated by GSK3B. Phosphorylation by GSK3B may negatively regulate binding to microtubule lattices in lamella. Isoform 2 is phosphorylated on Ser-241. |
| Function | Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. |
| Protein Name | Clip-Associating Protein 2Cytoplasmic Linker-Associated Protein 2 |
| Database Links | Reactome: R-MMU-141444Reactome: -MMU-2467813Reactome: -MMU-2500257Reactome: -MMU-5663220Reactome: -MMU-68877Reactome: -MMU-9648025 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeChromosomeCentromereKinetochoreSpindleSpindle PoleGolgi ApparatusTrans-Golgi NetworkCell MembraneCell ProjectionRuffle MembraneLocalizes To Microtubule Plus EndsLocalizes To CentrosomesKinetochores And The Mitotic Spindle From PrometaphaseSubsequently Localizes To The Spindle Midzone From Anaphase And To The Midbody From TelophaseIn Migrating Cells Localizes To The Plus Ends Of Microtubules Within The Cell Body And To The Entire Microtubule Lattice Within The LamellaLocalizes To The Cell Cortex And This Requires Erc1 And Phldb2Also Localizes To Meiotic Spindle PolesThe Memo1-Rhoa-Diaph1 Signaling Pathway Controls Localization Of The Phosphorylated Form To The Cell Membrane |
| Alternative Antibody Names | Anti-Clip-Associating Protein 2 antibodyAnti-Cytoplasmic Linker-Associated Protein 2 antibodyAnti-Clasp2 antibodyAnti-Kiaa0627 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
