| Host: |
Rat |
| Applications: |
WB/ICC |
| Reactivity: |
Chicken/Human/Mouse/Rat/Xenopus |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rat monoclonal antibody anti-CLASP1 (C-Term) is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
KT66 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2a |
| Formulation: |
PBS + 0.05% NaN3 |
| Purification: |
Protein G Purified |
| Dilution Range: |
WB 1:500IHCICC 1:100IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Clasp1 |
| Gene ID: |
76707 |
| Uniprot ID: |
CLAP1_MOUSE |
| Immunogen Region: |
C-Term |
| Specificity: |
The antibody detects a 161 kDa* protein corresponding to the molecular mass of CLASP1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects CLASP1 by immunocytochemistry in HeLa cells. |
| Immunogen: |
Clone KT66 was generated from a GST fusion protein containing the C-terminus of mouse CLASP1. This sequence is highly conserved in human, rat, chicken, and fish CLASP1, and has low homology to CLASP2. |
| Tissue Specificity | Highly expressed in brain and heart and at lower levels in kidney, lung, skeletal muscle and testis. |
| Function | Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. |
| Protein Name | Clip-Associating Protein 1Cytoplasmic Linker-Associated Protein 1 |
| Database Links | Reactome: R-MMU-141444Reactome: -MMU-2467813Reactome: -MMU-2500257Reactome: -MMU-2565942Reactome: -MMU-380259Reactome: -MMU-380270Reactome: -MMU-380284Reactome: -MMU-380320Reactome: -MMU-5620912Reactome: -MMU-5663220Reactome: -MMU-68877Reactome: -MMU-8854518Reactome: -MMU-9648025 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeChromosomeCentromereKinetochoreSpindleGolgi ApparatusTrans-Golgi NetworkLocalizes To Microtubule Plus EndsLocalizes To CentrosomesKinetochores And The Mitotic Spindle From PrometaphaseSubsequently Localizes To The Spindle Midzone From Anaphase And To The Midbody From TelophaseIn Migrating Cells Localizes To The Plus Ends Of Microtubules Within The Cell Body And To The Entire Microtubule Lattice Within The LamellaLocalizes To The Cell Cortex And This Requires Erc1 And Phldb2 |
| Alternative Antibody Names | Anti-Clip-Associating Protein 1 antibodyAnti-Cytoplasmic Linker-Associated Protein 1 antibodyAnti-Clasp1 antibodyAnti-Kiaa0622 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
