Anti-CHTOP antibody [7C4] (STJA0032589)
SPECIFICATIONS
ClonalityMonoclonal
HostRat
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenThe C-terminal fragment of mouse CHTOP
General Information
| Short Description | Rat monoclonal anti-CHTOP for use in WB, IHC-F, IHC-P, ICC, IF and IP in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF/IP |
| Host | Rat |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 7C4 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Chtop |
| Gene ID | 66511 |
| Uniprot ID | CHTOP_MOUSE |
| Immunogen | The C-terminal fragment of mouse CHTOP |
Additional Info
| Tissue Specificity | Broadly expressed with highest levels found in thymus, spleen, and lymph nodes. Expressed in an erythroid progenitor cell line derived from fetal liver. |
| Post Translational Modifications | Asymmetrically methylated by PRMT1. Symmetrically methylated by PRMT5. |
| Function | Plays an important role in the ligand-dependent activation of estrogen receptor target genes. May play a role in the silencing of fetal globin genes. Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Required for the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC methylates H4R3 and transactivates genes involved in glioblastomagenesis. |
| Protein Name | Chromatin Target Of Prmt1 ProteinFriend Of Prmt1 ProteinSmall Arginine- And Glycine-Rich ProteinSrag |
| Database Links | Reactome: R-MMU-159236Reactome: -MMU-72187Reactome: -MMU-73856 |
| Cellular Localisation | NucleusNucleolusNucleoplasmNucleus SpeckleMostly Associated With Facultative HeterochromatinLocalizes To Regions Surrounding Nuclear Speckles Known As Perispeckles In Which Trex Complex Assembly Seems To Occur |
| Alternative Antibody Names | Anti-Chromatin Target Of Prmt1 Protein antibodyAnti-Friend Of Prmt1 Protein antibodyAnti-Small Arginine- And Glycine-Rich Protein antibodyAnti-Srag antibodyAnti-Chtop antibodyAnti-Fop antibodyAnti-MNCb-1706 antibody |
Information sourced from Uniprot.org