| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CHRNA7 |
| Gene ID: | 1139/89832 |
| Uniprot ID: | ACHA7_HUMAN |
| Immunogen Region: | 23-230 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-230 of human CHRNA7 (NP_001177384.1). |
| Immunogen Sequence: | ASPPSTPPWDPGHIPGASVR PAPGPVSLQGEFQRKLYKEL VKNYNPLERPVANDSQPLTV YFSLSLLQIMDVDEKNQVLT TNIWLQMSWTDHYLQWNVSE YPGVKTVRFPDGQIWKPDIL LYNSADERFDATFHTNVLVN SSGHCQYLPPGIFKSSCYID VRWFPFDVQHCKLKFGSWSY GGWSLDLQMQEADISGYIPN GEWDLVGI |
| Post Translational Modifications | Glycosylations at Asn-46, Asn-90 and Asn-133 are essential for TMEM35A/NACHO-mediated proper subunit assembly and trafficking to the cell membrane. |
| Function | After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. |
| Protein Name | Neuronal Acetylcholine Receptor Subunit Alpha-7 |
| Database Links | Reactome: R-HSA-629594 |
| Cellular Localisation | Postsynaptic Cell MembraneMulti-Pass Membrane ProteinCell MembraneTmem35a/Nacho Promotes Its Trafficking To The Cell MembraneRic3 Promotes Its Trafficking To The Cell Membrane |
| Alternative Antibody Names | Anti-Neuronal Acetylcholine Receptor Subunit Alpha-7 antibodyAnti-CHRNA7 antibodyAnti-NACHRA7 antibody |
Information sourced from Uniprot.org