| Short Description : | Rabbit polyclonal CHRNA5 (23-240) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CHRNA5 |
| Gene ID: | 1138 |
| Uniprot ID: | ACHA5_HUMAN |
| Immunogen Region: | 23-240 |
| Immunogen Sequence: | RCGLAGAAGGAQRGLSEPSS IAKHEDSLLKDLFQDYERWV RPVEHLNDKIKIKFGLAISQ LVDVDEKNQLMTTNVWLKQE WIDVKLRWNPDDYGGIKVIR VPSDSVWTPDIVLFDNADGR FEGTSTKTVIRYNGTVTWTP PANYKSSCTIDVTFFPFDLQ NCSMKFGSWTYDGSQVDIIL EDQDVDKRDFFDNGEWEIVS ATGSKGNRTDSCCWYPYV |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-240 of human CHRNA5 (NP_000736.2). |
| Function | Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators. Has an accessory rather than functional role and is only able to form functional nAChRs when co-assembled with another beta subunit. Participates in pentameric assemblies along with CHRNA3, CHRNA4, CHRNB2 and CHRNB4. Increases receptor sensitivity to acetylcholine and nicotine when associated with CHRNA4 and CHRNB2. Plays a role in nicotine addiction. |
| Protein Name | Neuronal Acetylcholine Receptor Subunit Alpha-5 |
| Database Links | Reactome: R-HSA-629594Reactome: R-HSA-629597 |
| Cellular Localisation | Synaptic Cell MembraneMulti-Pass Membrane ProteinCell Membrane |
| Alternative Antibody Names | Anti-Neuronal Acetylcholine Receptor Subunit Alpha-5 antibodyAnti-CHRNA5 antibodyAnti-NACHRA5 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MAPKAPK3 knockout (KO) HeLa cells, using [KO Validated] MAPKAPK3 Rabbit polyclonal antibody (STJ11100068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89138/357587/STJ11100068_1__03762.1713121722.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MAPKAPK3 knockout (KO) HeLa cells, using [KO Validated] MAPKAPK3 Rabbit polyclonal antibody (STJ11100068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
