| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-CHRM2 (352-364) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 50kDa band observed in Mouse Brain lysates (calculated MW of 51.5kDa according to Mouse NP_987076.2). Recommended concentration: 0.5-2µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CHRM2 |
| Gene ID: | 1129 |
| Uniprot ID: | ACM2_HUMAN |
| Immunogen Region: | 352-364 |
| Accession Number: | NP_000730.1 |
| Specificity: | Reported variants represent identical protein: NP_001006628.1, NP_001006633.1, NP_001006627.1, NP_000730.1, NP_001006629.1, NP_001006630.1, NP_001006631.1, NP_001006632.1. |
| Immunogen Sequence: | QNGDEKQNIVARK |
| Function | The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3).this then triggers calcium ion release into the cytosol. |
| Protein Name | Muscarinic Acetylcholine Receptor M2 |
| Database Links | Reactome: R-HSA-390648 Reactome: R-HSA-418594 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Postsynaptic Cell Membrane Phosphorylation In Response To Agonist Binding Promotes Receptor Internalization |
| Alternative Antibody Names | Anti-Muscarinic Acetylcholine Receptor M2 antibody Anti-CHRM2 antibody |
Information sourced from Uniprot.org