• Immunofluorescence analysis of SH-SY5Y cells using CHRM1 Rabbit polyclonal antibody (STJ23130) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Neuro-2a cells using CHRM1 Rabbit polyclonal antibody (STJ23130) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using CHRM1 Rabbit polyclonal antibody (STJ23130) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CHRM1 antibody (STJ23130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHRM1 antibody (251-350) (STJ23130)

SKU:
STJ23130

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHRM1 (251-350) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHRM1
Gene ID: 1128
Uniprot ID: ACM1_HUMAN
Immunogen Region: 251-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 251-350 of human CHRM1 (NP_000729.2).
Immunogen Sequence: SPETPPGRCCRCCRAPRLLQ AYSWKEEEEEDEGSMESLTS SEGEEPGSEVVIKMPMVDPE AQAPTKQPPRSSPNTVKRPT KKGRDRAGKGQKPRGKEQLA
Function The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Protein Name Muscarinic Acetylcholine Receptor M1
Database Links Reactome: R-HSA-390648
Reactome: R-HSA-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Alternative Antibody Names Anti-Muscarinic Acetylcholine Receptor M1 antibody
Anti-CHRM1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance