| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human/Mouse/Rat/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-CHMP5 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 28kDa band observed in lysates of K562 (calculated MW of 24.5kDa according to NP_057494.2). The observed molecular weight corresponds to earlier findings in literature with diffe |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
CHMP5 |
| Gene ID: |
51510 |
| Uniprot ID: |
CHMP5_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_057494.3 |
| Immunogen Sequence: |
NKDGVLVDEFGLPQ |
| Function | Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. |
| Protein Name | Charged Multivesicular Body Protein 5Chromatin-Modifying Protein 5Snf7 Domain-Containing Protein 2Vacuolar Protein Sorting-Associated Protein 60Vps60Hvps60 |
| Database Links | Reactome: R-HSA-162588Reactome: R-HSA-917729 |
| Cellular Localisation | CytoplasmCytosolEndosome MembranePeripheral Membrane ProteinMidbodyLocalizes To The Midbody Of Dividing CellsLocalized In Two Distinct Rings On Either Side Of The Flemming Body |
| Alternative Antibody Names | Anti-Charged Multivesicular Body Protein 5 antibodyAnti-Chromatin-Modifying Protein 5 antibodyAnti-Snf7 Domain-Containing Protein 2 antibodyAnti-Vacuolar Protein Sorting-Associated Protein 60 antibodyAnti-Vps60 antibodyAnti-Hvps60 antibodyAnti-CHMP5 antibodyAnti-C9orf83 antibodyAnti-SNF7DC2 antibodyAnti-CGI-34 antibodyAnti-HSPC177 antibodyAnti-PNAS-114 antibodyAnti-PNAS-2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
