| Host: |
Sheep |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat/Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Sheep polyclonal antibody anti-CHMP3 (N-Term) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Chmp3 |
| Gene ID: |
66700 |
| Uniprot ID: |
CHMP3_MOUSE |
| Immunogen Region: |
N-Term |
| Specificity: |
Specific for CHMP3. |
| Immunogen: |
A synthetic peptide from the n-terminal of human CHMP3 conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in rat and mouse. |
| Function | Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. |
| Protein Name | Charged Multivesicular Body Protein 3Chromatin-Modifying Protein 3Vacuolar Protein Sorting-Associated Protein 24 |
| Database Links | Reactome: R-MMU-1632852Reactome: -MMU-5620971Reactome: -MMU-917729Reactome: -MMU-9668328 |
| Cellular Localisation | CytoplasmCytosolMembraneLipid-AnchorEndosomeLate Endosome MembraneLocalizes To The Midbody Of Dividing Cells |
| Alternative Antibody Names | Anti-Charged Multivesicular Body Protein 3 antibodyAnti-Chromatin-Modifying Protein 3 antibodyAnti-Vacuolar Protein Sorting-Associated Protein 24 antibodyAnti-Chmp3 antibodyAnti-Vps24 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
