Western blot analysis of extracts of HepG2 cells, using CHD1L Rabbit monoclonal antibody (STJ11102913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHD1L antibody (700-800) [S3MR] (STJ11102913)

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STJ11102913
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHD1L
Gene ID: 9557
Uniprot ID: CHD1L_HUMAN
Immunogen Region: 700-800
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 700-800 of human CHD1L (NP_004275.).
Immunogen Sequence: GEESSAELDYQDPDATSLKY VSGDVTHPQAGAEDALIVHC VDDSGHWGRGGLFTALEKRS AEPRKIYELAGKMKDLSLGG VLLFPVDDKESRNKGQDLLA L
Tissue Specificity Frequently overexpressed in hepatomacellular carcinomas.
Function ATP-dependent chromatin remodeler that mediates chromatin-remodeling following DNA damage. Recruited to DNA damage sites through interaction with poly-ADP-ribose: specifically recognizes and binds histones that are poly-ADP-ribosylated on serine residues in response to DNA damage. Poly-ADP-ribose-binding activates the ATP-dependent chromatin remodeler activity, thereby regulating chromatin during DNA repair. Catalyzes nucleosome sliding away from DNA breaks in an ATP-dependent manner. Chromatin remodeling activity promotes PARP2 removal from chromatin.
Protein Name Chromodomain-Helicase-Dna-Binding Protein 1-Like
Amplified In Liver Cancer Protein 1
Database Links Reactome: R-HSA-5696395
Reactome: R-HSA-5696400
Cellular Localisation Nucleus
Chromosome
Localizes At Sites Of Dna Damage
Recruited By Histones H2b And H3 Poly-Adp-Ribosylated On 'Ser-6' And 'Ser-10'
Respectively (H2bs6adpr And H3s10adpr) By Parp1 Or Parp2
Alternative Antibody Names Anti-Chromodomain-Helicase-Dna-Binding Protein 1-Like antibody
Anti-Amplified In Liver Cancer Protein 1 antibody
Anti-CHD1L antibody
Anti-ALC1 antibody

Information sourced from Uniprot.org

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