• Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using Chalk5 Rabbit polyclonal antibody (STJ11104067) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3s.

Anti-Chalk5 antibody (1-140 aa) (STJ11104067)

SKU:
STJ11104067

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Host: Rabbit
Applications: WB/ELISA
Reactivity: O.sativa
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-140 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of Oryza sativa Chalk5. (Q75M03).
Immunogen Sequence: MALIGTVAAEVLIPLAAVIG ILFAVLQWYMVSRVAVPPHD GVGGAGKVERESDGGDGDGD GVDDEEDGVDYRGVEARCAE IQHAISVGATSFLMTEYKYL GAFMAAFAAVIFVSLGSVGR FSTSTEPCPYDAARRCRPAL
Background Chalk5 encodes a vacuolar H+-translocating pyrophosphatase influencing grain chalkiness in rice.

Information sourced from Uniprot.org

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