• Immunofluorescence analysis of RAW 264. 7 cells using Complement factor B Rabbit polyclonal antibody (STJ11105589) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of THP-1 cells using Complement factor B Rabbit polyclonal antibody (STJ11105589) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Complement factor B using Complement factor B Rabbit polyclonal antibody (STJ11105589) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-CFB antibody (26-764) (STJ11105589)

SKU:
STJ11105589

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Complement Factor B (26-764) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CFB
Gene ID: 629
Uniprot ID: CFAB_HUMAN
Immunogen Region: 26-764
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 26-764 of human Complement factor B (NP_001701.2).
Immunogen Sequence: TPWSLARPQGSCSLEGVEIK GGSFRLLQEGQALEYVCPSG FYPYPVQTRTCRSTGSWSTL KTQDQKTVRKAECRAIHCPR PHDFENGEYWPRSPYYNVSD EISFHCYDGYTLRGSANRTC QVNGRWSGQTAICDNGAGYC SNPGIPIGTRKVGSQYRLED SVTYHCSRGLTLRGSQRRTC QEGGSWSGTEPSCQDSFMYD TPQEVAEAFLSSLTETIEGV DAEDGHGPGEQQKRKIVLD
Function Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
Protein Name Complement Factor B
C3/C5 Convertase
Glycine-Rich Beta Glycoprotein
Gbg
Pbf2
Properdin Factor B Cleaved Into - Complement Factor B Ba Fragment - Complement Factor B Bb Fragment
Database Links Reactome: R-HSA-173736
Reactome: R-HSA-174577
Reactome: R-HSA-977606
Cellular Localisation Secreted
Alternative Antibody Names Anti-Complement Factor B antibody
Anti-C3/C5 Convertase antibody
Anti-Glycine-Rich Beta Glycoprotein antibody
Anti-Gbg antibody
Anti-Pbf2 antibody
Anti-Properdin Factor B Cleaved Into - Complement Factor B Ba Fragment - Complement Factor B Bb Fragment antibody
Anti-CFB antibody
Anti-BF antibody
Anti-BFD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance