Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4ยฐC overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-CERKL antibody (341-390 aa) (STJ92238)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CERKL at the amino acid range 341-390
STJ92238
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General Information

Short DescriptionRabbit polyclonal anti-Ceramide kinase-like protein (341-390 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/ELISA
HostRabbit
ReactivityHuman/Rat/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCERKL
Gene ID375298
Uniprot IDCERKL_HUMAN
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CERKL at the amino acid range 341-390
Immunogen Region341-390 aa
SpecificityCERKL Polyclonal Antibody detects endogenous levels of CERKL protein.

Additional Info

Post Translational Modifications Phosphorylated on serine residues.
Function Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.
Protein Name Ceramide Kinase-Like Protein
Database Links
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Enriched In Nucleoli
May Shuttle Between Nucleus And Cytoplasm
Isoform 5 Is Not Enriched In The Nucleoli
Isoform 2: Cytoplasm
Golgi Apparatus
Trans-Golgi Network
Endoplasmic Reticulum
Alternative Antibody Names Anti-Ceramide Kinase-Like Protein antibody
Anti-CERKL antibody

Information sourced from Uniprot.org

Citations

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