• Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from A549 cells, using CERKL Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using CERKL Polyclonal Antibody

Anti-CERKL antibody (341-390 aa) (STJ92238)

SKU:
STJ92238

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ceramide kinase-like protein (341-390 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CERKL
Gene ID: 375298
Uniprot ID: CERKL_HUMAN
Immunogen Region: 341-390 aa
Specificity: CERKL Polyclonal Antibody detects endogenous levels of CERKL protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CERKL at the amino acid range 341-390
Post Translational Modifications Phosphorylated on serine residues.
Function Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.
Protein Name Ceramide Kinase-Like Protein
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Enriched In Nucleoli
May Shuttle Between Nucleus And Cytoplasm
Isoform 5 Is Not Enriched In The Nucleoli
Isoform 2: Cytoplasm
Golgi Apparatus
Trans-Golgi Network
Endoplasmic Reticulum
Alternative Antibody Names Anti-Ceramide Kinase-Like Protein antibody
Anti-CERKL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance