Anti-Centromere protein F/mitosin antibody (C-Term) (STJ70246)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-Centromere protein F/mitosin (C-Term) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/IHC |
| Host | Goat |
| Reactivity | Human/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Not yet tested-our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 358kDa according to NP_057427. Therefore we c |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | CENPF |
| Gene ID | 1063 |
| Uniprot ID | CENPF_HUMAN |
| Accession Number | AAA82889.1; AAA82935.1; AAA86889.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | GLESKGSENCKVQ |
| Specificity | The immunizing peptide shows one mismatch with the corresponding sequence of the NCBI-curated isoform NP_057427.3. |
Additional Info
| Function | Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. |
| Protein Name | Centromere Protein FCenp-FAh AntigenKinetochore Protein CenpfMitosin |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-156711Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmPerinuclear RegionNucleus MatrixChromosomeCentromereKinetochoreCytoskeletonSpindleRelocalizes To The Kinetochore/Centromere (Coronal Surface Of The Outer Plate) And The Spindle During MitosisObserved In Nucleus During Interphase But Not In The NucleolusAt Metaphase Becomes Localized To Areas Including Kinetochore And Mitotic Apparatus As Well As CytoplasmBy TelophaseIs Concentrated Within The Intracellular Bridge At Either Side Of The Mid-Body |
| Alternative Antibody Names | Anti-Centromere Protein F antibodyAnti-Cenp-F antibodyAnti-Ah Antigen antibodyAnti-Kinetochore Protein Cenpf antibodyAnti-Mitosin antibodyAnti-CENPF antibody |
Information sourced from Uniprot.org