Anti-CENTB1/ACAP1 antibody (C-Term) (STJ70439)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-CENTB1/ACAP1 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80kDa band observed in Jurkat lysates (calculated MW of 81.5kDa according to NP_055531.1). Recommended for use at 0.3-1µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ACAP1 |
| Gene ID | 9744 |
| Uniprot ID | ACAP1_HUMAN |
| Accession Number | NP_055531.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | EKLSRRSHDLHTL |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. |
| Function | GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. |
| Protein Name | Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 1Centaurin-Beta-1Cnt-B1 |
| Cellular Localisation | Recycling Endosome MembranePeripheral Membrane ProteinCytoplasmic Side |
| Alternative Antibody Names | Anti-Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 1 antibodyAnti-Centaurin-Beta-1 antibodyAnti-Cnt-B1 antibodyAnti-ACAP1 antibodyAnti-CENTB1 antibodyAnti-KIAA0050 antibody |
Information sourced from Uniprot.org