| Short Description : | Rabbit polyclonal CENPK (1-269) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CENPK |
| Gene ID: | 64105 |
| Uniprot ID: | CENPK_HUMAN |
| Immunogen Region: | 1-269 |
| Immunogen Sequence: | MNQEDLDPDSTTDVGDVTNT EEELIRECEEMWKDMEECQN KLSLIGTETLTDSNAQLSLL IMQVKCLTAELSQWQKKTPE TIPLTEDVLITLGKEEFQKL RQDLEMVLSTKESKNEKLKE DLEREQRWLDEQQQIMESLN VLHSELKNKVETFSESRIFN ELKTKMLNIKEYKEKLLSTL GEFLEDHFPLPDRSVKKKKK NIQESSVNLITLHEMLEILI NRLFDVPHDPYVKISDSFW |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human CENPK (NP_071428.2). |
| Tissue Specificity | Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta. |
| Function | Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore. |
| Protein Name | Centromere Protein KCenp-KInterphase Centromere Complex Protein 37Protein Af-5alphaP33 |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-606279Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreLocalizes Exclusively In The CentromeresThe Cenpa-Cad Complex Is Probably Recruited On Centromeres By The Cenpa-Nac Complex |
| Alternative Antibody Names | Anti-Centromere Protein K antibodyAnti-Cenp-K antibodyAnti-Interphase Centromere Complex Protein 37 antibodyAnti-Protein Af-5alpha antibodyAnti-P33 antibodyAnti-CENPK antibodyAnti-ICEN37 antibodyAnti-FKSG14 antibody |
Information sourced from Uniprot.org
