| Host: | Rabbit |
| Applications: | IHC/IF |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Centromere protein F (2190-2270 aa) is suitable for use in Immunohistochemistry and Immunofluorescence research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC-P 1:50-300 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CENPF |
| Gene ID: | 1063 |
| Uniprot ID: | CENPF_HUMAN |
| Immunogen Region: | 2190-2270 aa |
| Specificity: | CENPF Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from the human protein at the amino acid range 2190-2270 |
| Function | Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. |
| Protein Name | Centromere Protein F Cenp-F Ah Antigen Kinetochore Protein Cenpf Mitosin |
| Database Links | Reactome: R-HSA-141444 Reactome: R-HSA-156711 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-5663220 Reactome: R-HSA-68877 Reactome: R-HSA-9648025 |
| Cellular Localisation | Cytoplasm Perinuclear Region Nucleus Matrix Chromosome Centromere Kinetochore Cytoskeleton Spindle Relocalizes To The Kinetochore/Centromere (Coronal Surface Of The Outer Plate) And The Spindle During Mitosis Observed In Nucleus During Interphase But Not In The Nucleolus At Metaphase Becomes Localized To Areas Including Kinetochore And Mitotic Apparatus As Well As Cytoplasm By Telophase Is Concentrated Within The Intracellular Bridge At Either Side Of The Mid-Body |
| Alternative Antibody Names | Anti-Centromere Protein F antibody Anti-Cenp-F antibody Anti-Ah Antigen antibody Anti-Kinetochore Protein Cenpf antibody Anti-Mitosin antibody Anti-CENPF antibody |
Information sourced from Uniprot.org