• Immunofluorescence analysis of L929 cells using CELF5 Rabbit polyclonal antibody (STJ11100436) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CELF5 Rabbit polyclonal antibody (STJ11100436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CELF5 antibody (1-45) (STJ11100436)

SKU:
STJ11100436

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CELF5 (1-45) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CELF5
Gene ID: 60680
Uniprot ID: CELF5_HUMAN
Immunogen Region: 1-45
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-45 of human CELF5 (NP_001166144.1).
Immunogen Sequence: MARLTESEARRQQQQLLQPR PSPVGSSGPEPPGGQPDGMK DLDAI
Tissue Specificity Expressed in brain.
Function RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA.
Protein Name Cugbp Elav-Like Family Member 5
Celf-5
Bruno-Like Protein 5
Cug-Bp- And Etr-3-Like Factor 5
Rna-Binding Protein Brunol-5
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Cugbp Elav-Like Family Member 5 antibody
Anti-Celf-5 antibody
Anti-Bruno-Like Protein 5 antibody
Anti-Cug-Bp- And Etr-3-Like Factor 5 antibody
Anti-Rna-Binding Protein Brunol-5 antibody
Anti-CELF5 antibody
Anti-BRUNOL5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance