Anti-CELF1 antibody (71-120 aa) (STJ92521)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CELF-1 at the amino acid range 71-120
General Information
| Short Description | Rabbit polyclonal anti-CUGBP Elav-like family member 1 (71-120 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CELF1 |
| Gene ID | 10658 |
| Uniprot ID | CELF1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human CELF-1 at the amino acid range 71-120 |
| Immunogen Region | 71-120 aa |
| Specificity | CUG-BP1 Polyclonal Antibody detects endogenous levels of CUG-BP1 protein. |
Additional Info
| Post Translational Modifications | Phosphorylated. Its phosphorylation status increases in senescent cells. |
| Function | RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts both as an activator and as a repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver. Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF2, negatively regulates the processing to mature miRNA. |
| Protein Name | Cugbp Elav-Like Family Member 1Celf-150 Kda Nuclear Polyadenylated Rna-Binding ProteinBruno-Like Protein 2Cug Triplet Repeat Rna-Binding Protein 1Cug-Bp1Cug-Bp- And Etr-3-Like Factor 1Deadenylation Factor Cug-BpEmbryo Deadenylation Element-Binding Protein HomologEden-Bp HomologRna-Binding Protein Brunol-2 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmRna-Binding Activity Is Detected In Both Nuclear And Cytoplasmic Compartments |
| Alternative Antibody Names | Anti-Cugbp Elav-Like Family Member 1 antibodyAnti-Celf-1 antibodyAnti-50 Kda Nuclear Polyadenylated Rna-Binding Protein antibodyAnti-Bruno-Like Protein 2 antibodyAnti-Cug Triplet Repeat Rna-Binding Protein 1 antibodyAnti-Cug-Bp1 antibodyAnti-Cug-Bp- And Etr-3-Like Factor 1 antibodyAnti-Deadenylation Factor Cug-Bp antibodyAnti-Embryo Deadenylation Element-Binding Protein Homolog antibodyAnti-Eden-Bp Homolog antibodyAnti-Rna-Binding Protein Brunol-2 antibodyAnti-CELF1 antibodyAnti-BRUNOL2 antibodyAnti-CUGBP antibodyAnti-CUGBP1 antibodyAnti-NAB50 antibody |
Information sourced from Uniprot.org