STJ70305 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-CECR2 antibody (C-Term) (STJ70305)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70305-100
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General Information

Short DescriptionGoat polyclonal antibody anti-CECR2 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/FC
HostGoat
ReactivityHuman/Dog
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
IF: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ยตg/ml.

FC:Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml

Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolCECR2
Gene ID27443
Uniprot IDCECR2_HUMAN
Accession NumberNP_001276976.1; NP_001276975.1
Immunogen RegionC-Term
Immunogen SequencePPHKPPTLPLDQS

Additional Info

Function Regulatory subunit of the ATP-dependent CERF-1 and CERF-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair. The complexes do not have the ability to slide mononucleosomes to the center of a DNA template. The CERF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the CERF-5 ISWI chromatin remodeling complex. Plays a role in various processes during development: required during embryogenesis for neural tube closure and inner ear development. In adults, required for spermatogenesis, via the formation of ISWI-type chromatin complexes. In histone-modifying complexes, CECR2 recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated. May also be involved through its interaction with LRPPRC in the integration of cytoskeletal network with vesicular trafficking, nucleocytosolic shuttling, transcription, chromosome remodeling and cytokinesis.
Protein Name Chromatin Remodeling Regulator Cecr2
Cat Eye Syndrome Critical Region Protein 2
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Chromatin Remodeling Regulator Cecr2 antibody
Anti-Cat Eye Syndrome Critical Region Protein 2 antibody
Anti-CECR2 antibody
Anti-KIAA1740 antibody

Information sourced from Uniprot.org

Citations

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