Western blot analysis of extracts of various cell lines, using CDX1 antibody (STJ28280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDX1 antibody (136-265) (STJ28280)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ28280
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General Information

Short DescriptionRabbit polyclonal CDX1 (136-265) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCDX1
Gene ID1044
Uniprot IDCDX1_HUMAN
Immunogen Region136-265
Immunogen SequenceMRRSVAAGGGGGSGKTRTKD KYRVVYTDHQRLELEKEFHY SRYITIRRKSELAANLGLTE RQVKIWFQNRRAKERKVNKK KQQQQQPPQPPMAHDITATP AGPSLGGLCPSNTSLLATSS PMPVKEEFLP
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 136-265 of human CDX1 (NP_001795.2).

Additional Info

Tissue Specificity Intestinal epithelium.
Function Plays a role in transcriptional regulation. Involved in activated KRAS-mediated transcriptional activation of PRKD1 in colorectal cancer (CRC) cells. Binds to the PRKD1 promoter in colorectal cancer (CRC) cells. Could play a role in the terminal differentiation of the intestine. Binds preferentially to methylated DNA.
Protein Name Homeobox Protein Cdx-1
Caudal-Type Homeobox Protein 1
Database Links
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Cdx-1 antibody
Anti-Caudal-Type Homeobox Protein 1 antibody
Anti-CDX1 antibody

Information sourced from Uniprot.org

Citations

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