| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:20-1:50 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDKN1B |
| Gene ID: | 1027 |
| Uniprot ID: | CDN1B_HUMAN |
| Immunogen Region: | 1-198 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human CDKN1B/p27KIP1 (NP_004055.1). |
| Immunogen Sequence: | MSNVRVSNGSPSLERMDARQ AEHPKPSACRNLFGPVDHEE LTRDLEKHCRDMEEASQRKW NFDFQNHKPLEGKYEWQEVE KGSLPEFYYRPPRPPKGACK VPAQESQDVSGSRPAAPLIG APANSEDTHLVDPKTDPSDS QTGLAEQCAGIRKRPATDDS STQNKRANRTEENVSDGSPN AGSVEQTPKKPGLRRRQT |
| Tissue Specificity | Expressed in kidney (at protein level). Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney. |
| Post Translational Modifications | Phosphorylated.phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF. Ubiquitinated.in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated.by a TRIM21-containing SCF(SKP2)-like complex.leads to its degradation. Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation. |
| Function | Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. |
| Protein Name | Cyclin-Dependent Kinase Inhibitor 1b Cyclin-Dependent Kinase Inhibitor P27 P27kip1 |
| Database Links | Reactome: R-HSA-187577 Reactome: R-HSA-198323 Reactome: R-HSA-2559582 Reactome: R-HSA-2559586 Reactome: R-HSA-5625900 Reactome: R-HSA-5674400 Reactome: R-HSA-6804116 Reactome: R-HSA-69202 Reactome: R-HSA-69231 Reactome: R-HSA-69563 Reactome: R-HSA-69656 Reactome: R-HSA-8849470 Reactome: R-HSA-9607240 Reactome: R-HSA-9617828 Reactome: R-HSA-9634638 Reactome: R-HSA-9661069 |
| Cellular Localisation | Nucleus Cytoplasm Endosome Nuclear And Cytoplasmic In Quiescent Cells Akt- Or Rsk-Mediated Phosphorylation On Thr-198 Binds 14-3-3 Translocates To The Cytoplasm And Promotes Cell Cycle Progression Mitogen-Activated Uhmk1 Phosphorylation On Ser-10 Also Results In Translocation To The Cytoplasm And Cell Cycle Progression Phosphorylation On Ser-10 Facilitates Nuclear Export Translocates To The Nucleus On Phosphorylation Of Tyr-88 And Tyr-89 Colocalizes At The Endosome With Snx6 This Leads To Lysosomal Degradation |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase Inhibitor 1b antibody Anti-Cyclin-Dependent Kinase Inhibitor P27 antibody Anti-P27kip1 antibody Anti-CDKN1B antibody Anti-KIP1 antibody Anti-p27 antibody |
Information sourced from Uniprot.org