![Western blot analysis of lysates from wild type (WT) and CDK8 knockout (KO) HeLa cells, using [KO Validated] CDK8 Rabbit polyclonal antibody (STJ27494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101169/388564/STJ27494_2__94088.1713158082.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CDK8 knockout (KO) HeLa cells, using [KO Validated] CDK8 Rabbit polyclonal antibody (STJ27494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of lysates from Jurkat cells, using [KO Validated] CDK8 Rabbit polyclonal antibody (STJ27494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101169/388565/STJ27494_3__83315.1713158083.jpg?c=1 "Western blot analysis of lysates from Jurkat cells, using [KO Validated] CDK8 Rabbit polyclonal antibody (STJ27494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
Anti-CDK8 antibody (365-464) (STJ27494)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CDK8 (365-464) antibody for use in WB, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDK8 |
| Gene ID | 1024 |
| Uniprot ID | CDK8_HUMAN |
| Immunogen Region | 365-464 |
| Immunogen Sequence | DDKGDKKNQQQQQGNNHTNG TGHPGNQDSSHTQGPPLKKV RVVPPTTTSGGLIMTSDYQR SNPHAAYPNPGPSTSQPQSS MGYSATSQQPPQYSHQTHRY |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 365-464 of human CDK8 (NP_001251.1). |
Additional Info
| Function | Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. |
| Protein Name | Cyclin-Dependent Kinase 8Cell Division Protein Kinase 8Mediator Complex Subunit Cdk8Mediator Of Rna Polymerase Ii Transcription Subunit Cdk8Protein Kinase K35 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-2122947Reactome: R-HSA-212436Reactome: R-HSA-2173796Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-381340Reactome: R-HSA-9833110Reactome: R-HSA-9841922 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 8 antibodyAnti-Cell Division Protein Kinase 8 antibodyAnti-Mediator Complex Subunit Cdk8 antibodyAnti-Mediator Of Rna Polymerase Ii Transcription Subunit Cdk8 antibodyAnti-Protein Kinase K35 antibodyAnti-CDK8 antibody |
Information sourced from Uniprot.org