Host: | Rabbit |
Applications: | WB/IP/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:500-1:2000 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | CDK8 |
Gene ID: | 1024 |
Uniprot ID: | CDK8_HUMAN |
Immunogen Region: | 365-464 |
Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 365-464 of human CDK8 (NP_001251.1). |
Immunogen Sequence: | DDKGDKKNQQQQQGNNHTNG TGHPGNQDSSHTQGPPLKKV RVVPPTTTSGGLIMTSDYQR SNPHAAYPNPGPSTSQPQSS MGYSATSQQPPQYSHQTHRY |
Function | Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. |
Protein Name | Cyclin-Dependent Kinase 8 Cell Division Protein Kinase 8 Mediator Complex Subunit Cdk8 Mediator Of Rna Polymerase Ii Transcription Subunit Cdk8 Protein Kinase K35 |
Database Links | Reactome: R-HSA-1989781 Reactome: R-HSA-2122947 Reactome: R-HSA-212436 Reactome: R-HSA-2173796 Reactome: R-HSA-2644606 Reactome: R-HSA-2894862 Reactome: R-HSA-381340 Reactome: R-HSA-9833110 |
Cellular Localisation | Nucleus |
Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 8 antibody Anti-Cell Division Protein Kinase 8 antibody Anti-Mediator Complex Subunit Cdk8 antibody Anti-Mediator Of Rna Polymerase Ii Transcription Subunit Cdk8 antibody Anti-Protein Kinase K35 antibody Anti-CDK8 antibody |
Information sourced from Uniprot.org