• Western blot analysis of1) Hela Cell, 2) Jurkat Cell, 3) Mouse Brain Tissue, 4) Rat Brain Tissue lysate using mouse monoclonal antibody diluted at 1:2,000.
  • Western blot analysis of1) Hela Cell, 2) Jurkat Cell, 3) Mouse Brain Tissue, 4) Rat Brain Tissue lysate using mouse monoclonal antibody diluted at 1:2,000.
  • Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-CDK5 antibody [3D1] (STJ196638)

SKU:
STJ196638

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Cyclin-dependent kinase 5 is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Monoclonal
Clone ID: 3D1
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
WB 1:1000-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDK5
Gene ID: 1020
Uniprot ID: CDK5_HUMAN
Specificity: This antibody detects endogenous levels of CDK5 at Human, Mouse, Rat
Immunogen: Synthesized peptide derived from the human CDK5
Post Translational Modifications Phosphorylation on Tyr-15 by ABL1 and FYN, and on Ser-159 by casein kinase 1 promotes kinase activity. By contrast, phosphorylation at Thr-14 inhibits activity. Phosphorylation at Ser-159 is essential for maximal catalytic activity.
Function Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3-type G1 cyclins. Phosphorylates SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM. Regulates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins. Negatively regulates the CACNA1B/CAV2.2 -mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals. Activated by interaction with CDK5R1 (p35) and CDK5R2 (p39), especially in postmitotic neurons, and promotes CDK5R1 (p35) expression in an autostimulation loop. Phosphorylates many downstream substrates such as Rho and Ras family small GTPases (e.g. PAK1, RAC1, RHOA, CDC42) or microtubule-binding proteins (e.g. MAPT/TAU, MAP2, MAP1B), and modulates actin dynamics to regulate neurite growth and/or spine morphogenesis. Phosphorylates also exocytosis associated proteins such as MCAM/MUC18, SEPT5, SYN1, and CDK16/PCTAIRE1 as well as endocytosis associated proteins such as DNM1, AMPH and SYNJ1 at synaptic terminals. In the mature central nervous system (CNS), regulates neurotransmitter movements by phosphorylating substrates associated with neurotransmitter release and synapse plasticity.synaptic vesicle exocytosis, vesicles fusion with the presynaptic membrane, and endocytosis. Promotes cell survival by activating anti-apoptotic proteins BCL2 and STAT3, and negatively regulating of JNK3/MAPK10 activity. Phosphorylation of p53/TP53 in response to genotoxic and oxidative stresses enhances its stabilization by preventing ubiquitin ligase-mediated proteasomal degradation, and induces transactivation of p53/TP53 target genes, thus regulating apoptosis. Phosphorylation of p35/CDK5R1 enhances its stabilization by preventing calpain-mediated proteolysis producing p25/CDK5R1 and avoiding ubiquitin ligase-mediated proteasomal degradation. During aberrant cell-cycle activity and DNA damage, p25/CDK5 activity elicits cell-cycle activity and double-strand DNA breaks that precedes neuronal death by deregulating HDAC1. DNA damage triggered phosphorylation of huntingtin/HTT in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Phosphorylation of PXN reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Negative regulator of Wnt/beta-catenin signaling pathway. Activator of the GAIT (IFN-gamma-activated inhibitor of translation) pathway, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.phosphorylates the linker domain of glutamyl-prolyl tRNA synthetase (EPRS) in a IFN-gamma-dependent manner, the initial event in assembly of the GAIT complex. Phosphorylation of SH3GLB1 is required for autophagy induction in starved neurons. Phosphorylation of TONEBP/NFAT5 in response to osmotic stress mediates its rapid nuclear localization. MEF2 is inactivated by phosphorylation in nucleus in response to neurotoxin, thus leading to neuronal apoptosis. APEX1 AP-endodeoxyribonuclease is repressed by phosphorylation, resulting in accumulation of DNA damage and contributing to neuronal death. NOS3 phosphorylation down regulates NOS3-derived nitrite (NO) levels. SRC phosphorylation mediates its ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. May regulate endothelial cell migration and angiogenesis via the modulation of lamellipodia formation. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-BMAL1 heterodimer in association with altered stability and subcellular distribution.
Protein Name Cyclin-Dependent Kinase 5
Cell Division Protein Kinase 5
Cyclin-Dependent-Like Kinase 5
Serine/Threonine-Protein Kinase Pssalre
Tau Protein Kinase Ii Catalytic Subunit
Tpkii Catalytic Subunit
Database Links Reactome: R-HSA-180024
Reactome: R-HSA-399956
Reactome: R-HSA-6804756
Reactome: R-HSA-8862803
Reactome: R-HSA-9031628
Reactome: R-HSA-9032845
Reactome: R-HSA-9768919
Reactome: R-HSA-983231
Cellular Localisation Isoform 1: Cytoplasm
Nucleus
Cell Membrane
Peripheral Membrane Protein
Perikaryon
Cell Projection
Lamellipodium
Growth Cone
Postsynaptic Density
Synapse
In Axonal Growth Cone With Extension To The Peripheral Lamellipodia
Under Neurotoxic Stress And Neuronal Injury Conditions
Cdk5r (P35) Is Cleaved By Calpain To Generate Cdk5r1 (P25) In Response To Increased Intracellular Calcium
The Elevated Level Of P25
When In Complex With Cdk5
Leads To Its Subcellular Misallocation As Well As Its Hyperactivation
Colocalizes With Ctnnd2 In The Cell Body Of Neuronal Cells
And With Ctnnb1 In The Cell-Cell Contacts And Plasma Membrane Of Undifferentiated And Differentiated Neuroblastoma Cells
Reversibly Attached To The Plasma Membrane In An Inactive Form When Complexed To Dephosphorylated P35 Or Cdk5r2 (P39)
P35 Phosphorylation Releases This Attachment And Activates Cdk5
Isoform 2: Nucleus
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 5 antibody
Anti-Cell Division Protein Kinase 5 antibody
Anti-Cyclin-Dependent-Like Kinase 5 antibody
Anti-Serine/Threonine-Protein Kinase Pssalre antibody
Anti-Tau Protein Kinase Ii Catalytic Subunit antibody
Anti-Tpkii Catalytic Subunit antibody
Anti-CDK5 antibody
Anti-CDKN5 antibody
Anti-PSSALRE antibody

Information sourced from Uniprot.org

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