![Immunohistochemistry analysis of CDK4 in paraffin-embedded human cervix cancer tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90192/359144/STJ11101279_IHC_7__63473.1713123555.jpg?c=1 "Immunohistochemistry analysis of CDK4 in paraffin-embedded human cervix cancer tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of CDK4 in paraffin-embedded human tonsil tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90192/359145/STJ11101279_IHC_8__69269.1713123557.jpg?c=1 "Immunohistochemistry analysis of CDK4 in paraffin-embedded human tonsil tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of CDK4 in paraffin-embedded human breast cancer tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90192/359146/STJ11101279_IHC_9__68187.1713123558.jpg?c=1 "Immunohistochemistry analysis of CDK4 in paraffin-embedded human breast cancer tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of CDK4 in paraffin-embedded human cervix cancer tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90192/359147/STJ11101279_IHC_10__15131.1713123559.jpg?c=1 "Immunohistochemistry analysis of CDK4 in paraffin-embedded human cervix cancer tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of CDK4 in paraffin-embedded human prostate tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90192/359148/STJ11101279_IHC_11__73427.1713123560.jpg?c=1 "Immunohistochemistry analysis of CDK4 in paraffin-embedded human prostate tissue using [KO Validated] CDK4 rabbit monoclonal antibody (STJ11101279) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.")
Anti-CDK4 antibody (204-303) [S9MR] (STJ11101279)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal CDK4 (204-303) antibody for use in WB, IHC-P and ELISA in human, mouse, rat and monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S9MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IHC-P:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDK4 |
| Gene ID | 1019 |
| Uniprot ID | CDK4_HUMAN |
| Immunogen Region | 204-303 |
| Immunogen Sequence | FAEMFRRKPLFCGNSEADQL GKIFDLIGLPPEDDWPRDVS LPRGAFPPRGPRPVQSVVPE MEESGAQLLLEMLTFNPHKR ISAFRALQHSYLHKDEGNPE |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 204-303 of human CDK4 (NP_000066.1). |
Additional Info
| Post Translational Modifications | Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B. |
| Function | Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
| Protein Name | Cyclin-Dependent Kinase 4Cell Division Protein Kinase 4Psk-J3 |
| Database Links | Reactome: R-HSA-187577Reactome: R-HSA-2559580Reactome: R-HSA-2559582Reactome: R-HSA-2559585Reactome: R-HSA-3214858Reactome: R-HSA-381340Reactome: R-HSA-69231Reactome: R-HSA-75815Reactome: R-HSA-8849470Reactome: R-HSA-8878166Reactome: R-HSA-912446Reactome: R-HSA-9616222Reactome: R-HSA-9630791Reactome: R-HSA-9630794Reactome: R-HSA-9632697Reactome: R-HSA-9632700Reactome: R-HSA-9661069Reactome: R-HSA-9754119 |
| Cellular Localisation | CytoplasmNucleusNucleus MembraneCytoplasmic When Non-ComplexedForms A Cyclin D-Cdk4 Complex In The Cytoplasm As Cells Progress Through G(1) PhaseThe Complex Accumulates On The Nuclear Membrane And Enters The Nucleus On Transition From G(1) To S PhaseAlso Present In Nucleoli And Heterochromatin LumpsColocalizes With Rb1 After Release Into The Nucleus |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 4 antibodyAnti-Cell Division Protein Kinase 4 antibodyAnti-Psk-J3 antibodyAnti-CDK4 antibody |
Information sourced from Uniprot.org