| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | SRM |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:12000IHC-P:1:200-1:800IF/ICC:1:200-1:800IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your sp |
| Gene Symbol: | CDK4 |
| Gene ID: | 1019 |
| Uniprot ID: | CDK4_HUMAN |
| Immunogen Region: | 204-303 aa |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 204-303 of human CDK4 (NP_000066.1). |
| Immunogen Sequence: | FAEMFRRKPLFCGNSEADQL GKIFDLIGLPPEDDWPRDVS LPRGAFPPRGPRPVQSVVPE MEESGAQLLLEMLTFNPHKR ISAFRALQHSYLHKDEGNPE |
| Post Translational Modifications | Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B. |
| Function | Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
| Protein Name | Cyclin-Dependent Kinase 4Cell Division Protein Kinase 4Psk-J3 |
| Database Links | Reactome: R-HSA-187577Reactome: R-HSA-2559580Reactome: R-HSA-2559582Reactome: R-HSA-2559585Reactome: R-HSA-3214858Reactome: R-HSA-381340Reactome: R-HSA-69231Reactome: R-HSA-75815Reactome: R-HSA-8849470Reactome: R-HSA-8878166Reactome: R-HSA-912446Reactome: R-HSA-9616222Reactome: R-HSA-9630791Reactome: R-HSA-9630794Reactome: R-HSA-9632697Reactome: R-HSA-9632700Reactome: R-HSA-9661069Reactome: R-HSA-9754119 |
| Cellular Localisation | CytoplasmNucleusNucleus MembraneCytoplasmic When Non-ComplexedForms A Cyclin D-Cdk4 Complex In The Cytoplasm As Cells Progress Through G(1) PhaseThe Complex Accumulates On The Nuclear Membrane And Enters The Nucleus On Transition From G(1) To S PhaseAlso Present In Nucleoli And Heterochromatin LumpsColocalizes With Rb1 After Release Into The Nucleus |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 4 antibodyAnti-Cell Division Protein Kinase 4 antibodyAnti-Psk-J3 antibodyAnti-CDK4 antibody |
Information sourced from Uniprot.org
![Immunoprecipitation analysis of 300 Mu g extracts of Hela cells using 3 Mu g [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180). Western blot was performed from the immunoprecipitate using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180) at a dilition of 1:1000.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/90102/358868/STJ11101180_1__04910.1713123234.jpg?c=1 "Immunoprecipitation analysis of 300 Mu g extracts of Hela cells using 3 Mu g [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180). Western blot was performed from the immunoprecipitate using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180) at a dilition of 1:1000.")
![Western blot analysis of various lysates using [KO Validated] CDK4 Rabbit pAb (STJ23055) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/99347/410985/STJ23055_1__60235.1744897543.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] CDK4 Rabbit pAb (STJ23055) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.")