Anti-CDH5 antibody (697-746 aa) (STJ96234)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CDH5 at the amino acid range 697-746
General Information
| Short Description | Rabbit polyclonal anti-Cadherin-5 (697-746 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDH5 |
| Gene ID | 1003 |
| Uniprot ID | CADH5_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human CDH5 at the amino acid range 697-746 |
| Immunogen Region | 697-746 aa |
| Specificity | VE-Cadherin Polyclonal Antibody detects endogenous levels of VE-Cadherin protein. |
Additional Info
| Post Translational Modifications | Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. O-glycosylated. |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1. Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Associates with CTNND1/p120-catenin to control CADH5 endocytosis. |
| Protein Name | Cadherin-57b4 AntigenVascular Endothelial CadherinVe-CadherinCd Antigen Cd144 |
| Database Links | Reactome: R-HSA-418990Reactome: R-HSA-5218920Reactome: R-HSA-9856530 |
| Cellular Localisation | Cell JunctionAdherens JunctionCell MembraneSingle-Pass Type I Membrane ProteinCytoplasmFound At Cell-Cell Boundaries And Probably At Cell-Matrix BoundariesKrit1 And Cdh5 Reciprocally Regulate Their Localization To Endothelial Cell-Cell Junctions |
| Alternative Antibody Names | Anti-Cadherin-5 antibodyAnti-7b4 Antigen antibodyAnti-Vascular Endothelial Cadherin antibodyAnti-Ve-Cadherin antibodyAnti-Cd Antigen Cd144 antibodyAnti-CDH5 antibody |
Information sourced from Uniprot.org