![Immunohistochemistry analysis of N-Cadherin in paraffin-embedded Mouse liver using [KO Validated] N-Cadherin Rabbit polyclonal antibody (STJ11104277) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176921/367953/STJ11104277_2__79971.1713133487.jpg?c=1 "Immunohistochemistry analysis of N-Cadherin in paraffin-embedded Mouse liver using [KO Validated] N-Cadherin Rabbit polyclonal antibody (STJ11104277) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of N-Cadherin in paraffin-embedded Human colon carcinoma using [KO Validated] N-Cadherin Rabbit polyclonal antibody (STJ11104277) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176921/367954/STJ11104277_3__05159.1713133488.jpg?c=1 "Immunohistochemistry analysis of N-Cadherin in paraffin-embedded Human colon carcinoma using [KO Validated] N-Cadherin Rabbit polyclonal antibody (STJ11104277) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and N-Cadherin knockout (KO) HeLa cells, using [KO Validated] N-Cadherin Rabbit polyclonal antibody (STJ11104277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176921/367955/STJ11104277_4__71967.1713133489.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and N-Cadherin knockout (KO) HeLa cells, using [KO Validated] N-Cadherin Rabbit polyclonal antibody (STJ11104277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from 293T cells, using [KO Validated] N-Cadherin Rabbit polyclonal antibody (STJ11104277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176921/367956/STJ11104277_5__33341.1713133489.jpg?c=1 "Western blot analysis of lysates from 293T cells, using [KO Validated] N-Cadherin Rabbit polyclonal antibody (STJ11104277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from HeLa cells, using [KO Validated] N-Cadherin Rabbit polyclonal antibody (STJ11104277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176921/367957/STJ11104277_6__79814.1713133490.jpg?c=1 "Western blot analysis of lysates from HeLa cells, using [KO Validated] N-Cadherin Rabbit polyclonal antibody (STJ11104277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of various lysates using [KO Validated] N-Cadherin Rabbit polyclonal antibody (STJ11104277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176921/367958/STJ11104277_7__42742.1713133491.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] N-Cadherin Rabbit polyclonal antibody (STJ11104277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
| Host: | Rabbit |
| Applications: | WB/IHC-P/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDH2 |
| Gene ID: | 1000 |
| Uniprot ID: | CADH2_HUMAN |
| Immunogen Region: | 807-906 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 807-906 of human N-Cadherin (NP_001783.2). |
| Immunogen Sequence: | RRMDERPIHAEPQYPVRSAA PHPGDIGDFINEGLKAADND PTAPPYDSLLVFDYEGSGST AGSLSSLNSSSSGGEQDYDY LNDWGPRFKKLADMYGGGDD |
| Post Translational Modifications | Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa N-terminal soluble fragment and a 45 kDa membrane-bound C-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence. May be phosphorylated by OBSCN. |
| Function | Calcium-dependent cell adhesion protein.preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells. Required for proper neurite branching. Required for pre- and postsynaptic organization. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. |
| Protein Name | Cadherin-2 Cdw325 Neural Cadherin N-Cadherin Cd Antigen Cd325 |
| Database Links | Reactome: R-HSA-381426 Reactome: R-HSA-418990 Reactome: R-HSA-525793 Reactome: R-HSA-8957275 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Sarcolemma Cell Junction Cell Surface Desmosome Adherens Junction Colocalizes With Tmem65 At The Intercalated Disk In Cardiomyocytes Colocalizes With Obscn At The Intercalated Disk And At Sarcolemma In Cardiomyocytes |
| Alternative Antibody Names | Anti-Cadherin-2 antibody Anti-Cdw325 antibody Anti-Neural Cadherin antibody Anti-N-Cadherin antibody Anti-Cd Antigen Cd325 antibody Anti-CDH2 antibody Anti-CDHN antibody Anti-NCAD antibody |
Information sourced from Uniprot.org