• Western blot analysis of extracts of various cell lines, using Cadherin 16 antibody (STJ110192) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of rat kidney using Cadherin 16 Polyclonal Antibody (STJ110192) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat kidney using Cadherin 16 antibody (STJ110192) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Cadherin 16 antibody (STJ110192) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Cadherin 16 antibody (STJ110192) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Cadherin 16 antibody (STJ110192) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat kidney using Cadherin 16 Polyclonal Antibody (STJ110192) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Cadherin 16 antibody (STJ110192) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDH16 antibody (19-230) (STJ110192)

SKU:
STJ110192

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDH16 (19-230) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDH16
Gene ID: 1014
Uniprot ID: CAD16_HUMAN
Immunogen Region: 19-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-230 of human Cadherin 16 (NP_004053.1).
Immunogen Sequence: KAQPAELSVEVPENYGGNFP LYLTKLPLPREGAEGQIVLS GDSGKATEGPFAMDPDSGFL LVTRALDREEQAEYQLQVTL EMQDGHVLWGPQPVLVHVKD ENDQVPHFSQAIYRARLSRG TRPGIPFLFLEASDRDEPGT ANSDLRFHILSQAPAQPSPD MFQLEPRLGALALSPKGSTS LDHALERTYQLLVQVKDMGD QASGHQATATVE
Tissue Specificity Kidney specific.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types.
Protein Name Cadherin-16
Kidney-Specific Cadherin
Ksp-Cadherin
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cadherin-16 antibody
Anti-Kidney-Specific Cadherin antibody
Anti-Ksp-Cadherin antibody
Anti-CDH16 antibody
Anti-UNQ695 antibody
Anti-PRO1340 antibody

Information sourced from Uniprot.org

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