• Immunofluorescence analysis of paraffin-embedded mouse kidney using E-Cadherin Mouse monoclonal antibody (STJ11100105) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using E-Cadherin Mouse monoclonal antibody (STJ11100105) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDH1 antibody (373-622) [S5MM] (STJ11100105)

SKU:
STJ11100105

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Host: Mouse
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-E-Cadherin (373-622) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MM
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDH1
Gene ID: 999
Uniprot ID: CADH1_HUMAN
Immunogen Region: 373-622
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 373-622 of human E-Cadherin (P12830).
Immunogen Sequence: PIFNPTTYKGQVPENEANVV ITTLKVTDADAPNTPAWEAV YTILNDDGGQFVVTTNPVNN DGILKTAKGLDFEAKQQYIL HVAVTNVVPFEVSLTTSTAT VTVDVLDVNEAPIFVPPEKR VEVSEDFGVGQEITSYTAQE PDTFMEQKITYRIWRDTANW LEINPDTGAISTRAELDRED FEHVKNSTYTALIIATDNGS PVATGTGTLLLILSDVNDNA PIPEPRTIFFCERNPKPQV
Tissue Specificity Non-neural epithelial tissues.
Post Translational Modifications During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation. N-glycosylation at Asn-637 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location. Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754. O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Thr-285 and Thr-509 are O-mannosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3. (Microbial infection) Cleaved by S.pyogenes SpeB protease.leading to its degradation. Degradation by SpeB promotes bacterial translocation across the host epithelial barrier.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. (Microbial infection) Serves as a receptor for Listeria monocytogenes.internalin A (InlA) binds to this protein and promotes uptake of the bacteria.
Protein Name Cadherin-1
Cam 120/80
Epithelial Cadherin
E-Cadherin
Uvomorulin
Cd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-198933
Reactome: R-HSA-216083
Reactome: R-HSA-351906
Reactome: R-HSA-418990
Reactome: R-HSA-5626467
Reactome: R-HSA-8876493
Reactome: R-HSA-9823730
Cellular Localisation Cell Junction
Adherens Junction
Cell Membrane
Single-Pass Type I Membrane Protein
Endosome
Golgi Apparatus
Trans-Golgi Network
Colocalizes With Dlgap5 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells
Anchored To Actin Microfilaments Through Association With Alpha-
Beta- And Gamma-Catenin
Sequential Proteolysis Induced By Apoptosis Or Calcium Influx
Results In Translocation From Sites Of Cell-Cell Contact To The Cytoplasm
Colocalizes With Rab11a Endosomes During Its Transport From The Golgi Apparatus To The Plasma Membrane
Alternative Antibody Names Anti-Cadherin-1 antibody
Anti-Cam 120/80 antibody
Anti-Epithelial Cadherin antibody
Anti-E-Cadherin antibody
Anti-Uvomorulin antibody
Anti-Cd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 antibody
Anti-CDH1 antibody
Anti-CDHE antibody
Anti-UVO antibody

Information sourced from Uniprot.org

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