| Host: |
Rabbit |
| Applications: |
WB/ELISA/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Serine/threonine-protein kinase MRCK alpha (580-660) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-p 1:50-300ELISA 2000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CDC42BPA |
| Gene ID: |
8476 |
| Uniprot ID: |
MRCKA_HUMAN |
| Immunogen Region: |
580-660 |
| Specificity: |
MRCK Alpha Polyclonal Antibody detects endogenous levels of MRCK Alpha protein. |
| Immunogen: |
Synthesized peptide derived from the Internal region of human MRCK Alpha. AA range: 580-660 |
| Tissue Specificity | Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver. |
| Post Translational Modifications | Proteolytically cleaved by caspases upon apoptosis induction. The cleavage at Asp-478 by CASP3 increases its kinase activity (in vitro). |
| Function | Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation. Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells. |
| Protein Name | Serine/Threonine-Protein Kinase Mrck AlphaCdc42-Binding Protein Kinase AlphaDmpk-Like AlphaMyotonic Dystrophy Kinase-Related Cdc42-Binding Kinase AlphaMrck AlphaMyotonic Dystrophy Protein Kinase-Like Alpha |
| Database Links | Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013406Reactome: R-HSA-9013409 |
| Cellular Localisation | CytoplasmCell ProjectionLamellipodiumDisplays A Dispersed Punctate Distribution And Concentrates Along The Cell PeripheryEspecially At The Leading Edge And Cell-Cell JunctionThis Concentration Is Ph-Domain DependentLocalizes In The Lamellipodium In A Fam89b/Lrap25-Dependent Manner |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Mrck Alpha antibodyAnti-Cdc42-Binding Protein Kinase Alpha antibodyAnti-Dmpk-Like Alpha antibodyAnti-Myotonic Dystrophy Kinase-Related Cdc42-Binding Kinase Alpha antibodyAnti-Mrck Alpha antibodyAnti-Myotonic Dystrophy Protein Kinase-Like Alpha antibodyAnti-CDC42BPA antibodyAnti-KIAA0451 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
